The Effect Of Substrate on Enzyme concentration

h Wear goggles, apron and gloves to be safe in case of spillage or accidental contact with clothing.

h Upon contact with solution it is advisable to wash transfer or affected body part immediately. Be careful as it burns.

h If breakage of furnishs/beaker occurs, clean up immediately.

h In hallow not to confuse test undergrounds containing water and heat content peroxide, label each of them clearly

h Remember to wash hands after handling yeast suspension

FAIR TEST

To ensure that a evenhandedly test is carried come on the following must be observed

h Make sure fixed variables are not altered

h Make sure that when the volume of yeast and water is changed and accordingly added to hydrogen peroxide the total volume is 8 cm3.

h Make sure the volume of hydrogen peroxide in all the experiments is kept constant at 3 cm3

h Ensure the clamp is firmly in place so there are mishaps and no interruptions

h Carry out experiment more than once and then find an second-rate this should eliminate anomalies

h Mix the reagents and start the clock at the same time, so that the correct value will be got.

h Make sure air bubbles arent formed in front the reaction begins by using the bung

h Try as much as possible to avoid parallax error.

FINAL CHOICE OF EQUIPMENT

The slew up of my apparatus will be similar to my preliminary examination experiment, just a few changes have been made and a few things added:

h Clamp (x1) (indicated by arrow)

h surface retort stand (x1)

h Electronic timer (recording to 1 sec intervals) (x1)

h Beaker (500 cm3) (x1)

h Micro test tube to fit into side-arm boiling tube

h Large side-arm boiling tube and bung fitted with delivery tube to fit (x5)

h gradational measuring cylinder...

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